Lipid Metabolism & Intracellular Trafficking

The endo-lysosomal network forms a highly dynamic network of interacting membranous compartments. This network is involved in regulating various cellular processes, including lipid metabolism. Our primary focus is on understanding how the endo-lysosomal system controls lipid metabolism, and how this knowledge can be used to combat lipid-related diseases, like atherosclerosis and non-alcoholic fatty liver disease.

Methodologies

To achieve our research goals, we employ cutting edge technologies:

• CRISPR gene editing technology
• Transgenic mouse models
• Primary cell cultures
• Biochemical studies
• Advanced imaging techniques  

Key Research Areas

1. Intracellular lipid trafficking, with a focus on cholesterol
2. Endocytic pathways controlling cholesterol uptake
3. Transport of internalized cholesterol between intracellular compartments
4. Mechanisms of how cellular cholesterol efflux 
5. Impact metabolic changes on endo-lysosomal network function

Research Focus

We concentrate on:

• Unraveling the mechanisms by which the endo-lysosomal system regulates plasma and liver lipid levels
• Exploring how these mechanisms influence the risk of CVD and fatty liver disease
• Investigating the intracellular trafficking machinery of LDL receptors (LDLR), key determinants of plasma LDL cholesterol concentrations

Potential Impact

By elucidating these intricate cellular processes, our work aims to:

1. Enhance our understanding of lipid homeostasis
2. Facilitate the development of novel therapeutic strategies to reduce plasma and liver lipid levels
3. Contribute to the prevention and treatment of CVD and fatty liver disease

Through our innovative research, we strive to make significant contributions to cardiovascular health and improve patient outcomes in the face of rising CVD prevalence.

Collaborations

We work closely with the team of Jan Albert Kuivenhoven, sharing PhD students and postdocs to foster interdisciplinary research.

Our team has established a state-of-the-art mouse facility to create innovative mouse models, enhancing our fundamental and biomedical research capabilities at UMCG.

Transgenic Mouse Clinic for Ageing Research