The aim of this thesis of Annick van Furth was to advance the understanding and preservation of renal energy metabolism through the application of nutritional modifications during ex vivo normothermic machine perfusion (NMP). The research was predominantly conducted using porcine kidneys, employing two experimental models: precision-cut kidney slices and normothermic machine perfusion. Various dietary interventions were explored, including a ketogenic diet, different macronutrient compositions, lactate supplementation, and fasting. Additionally, metabolomics were used to investigate the intricate metabolic processes occurring during NMP. The impact of different hemoglobin concentrations on renal function during NMP was also examined to ensure adequate oxygenation of porcine kidneys. Furthermore, human kidneys were used to evaluate energy metabolism and assess lactate as a potential viability assessment tool prior to transplantation. By deepening the understanding of renal nutritional requirements and optimizing preservation strategies before transplantation, this research aims to contribute to improved outcomes in renal transplantation.
Annick van Furth is part of PRECISION.
