For researchers preparing their own NGS sequencing libraries (e.g. RNA-seq, ATAC-seq, ChIP-seq, Sure-Seq, and/or single cell omics variants), we offer standalone next generation sequencing as a service using an Illumina NextSeq 500 or MiSeq sequencer.
- NextSeq500 High Output runs:
- output 25 - 120 Gb
- ~ 400 million clusters
- Single-end run options for 75 or 150 cycles
- Paired-end run options for 40, 75, or 150 cycles
- NextSeq500 Mid Output runs:
- output 16 - 39 Gb
- ~ 130 million clusters
- Single-end run options for 150 cycles
- Paired-end run options for 75 or 150 cycles
- MiSeq, divers sequencing kits:
- output 300 Mb - 15 Gb
- Single-end run options for 36, 75, 150 or 300 cycles
- Paired-end run options for 75 or 150 or 300 cycles
We accept individual or pooled libraries for which we can perform the quantity and quality assessment, and pooling in case of individual libraries before sequencing. Alternatively, the researcher can perform these assessments him- or herself but is obliged to provide QC information to determine if the quality of the superpool meets our requirements.
Apart from our in-house sequencing service, we offer preliminary data analysis including demultiplexing of the raw sequencing data, subsequent alignment to the reference genome of interest and generation of gene count lists using automated bioinformatics pipelines. Furthermore, we can help you with downstream analysis of small-scale omics projects. However, if you need help with more advanced data analysis, we will refer you to the Genomics Coordination Center. Please contact us for more information using the intake form.