Interfaculty Mass Spectrometry Center (IMSC)

Mass spectrometry services and infrastructure for LC-MS(MS) analysis, proteomics and metabolomics Facility
We supports all research groups of the University of Groningen, University Medical Center Groningen and external academic and commercial customers. In their efforts to characterise or quantify molecules of chemical, biological and pharmaceutical interest.

Our facility has standard protocols for proteomics analysis and offers scientific expertise in collaborative research projects aiming to develop new sample preparation methods, analytical techniques and specific bioinformatics analysis approaches for proteomics and metabolomics analysis.

We support the full process of sample preparation, LC-MS analysis and data analysis and provide expertise on experimental design, data processing and analysis and multi-omics data integration.

The available equipment includes:

A wide range of mass spectrometers (MS):

  • Orbitraps
  • QTOFs
  • Triple quadrupoles

Sample preparation and separation systems:

  • HPLC
  • UPLC
  • NanoLC

Free available and commercial data processing tools:

  • MaxQuant
  • PEAKS
  • Proteome Discoverer
  • Spectronaut
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Service requests

All MS instruments and associated equipment are operated, supported, and supervised by IMSC technicians.

In most cases samples are submitted to the IMSC for analysis including data analysis. The standard user fee for RUG and UMCG customers is 35 euro per hour.

  • Samples for MS analysis can be submitted to the IMSC facility, at the location in the ERIBA building (lab 3226.0650) or mailed to the IMSC address.

    Please contact us before sending samples to discuss sample preparation and analysis details.

  • Experts support

    All MS instruments and associated equipment are operated, supported and supervised by one or more IMSC technicians. The technicians all have broad MS experience and often have in-depth knowledge on specific subjects or methods. The general policy of the IMSC is to provide high-level service to every scientist and research group from the RuG and UMCG to the best of our abilities.

    Costs intern and external customers

    Exploitation of the IMSC, notably repair and maintenance of MS equipment, and costs of MS-specific consumables is covered by user fees (standard fee is 35 euro per hour, per Jan 1st 2019). For external customers (non-RuG or UMCG) overhead and personnel costs are added (details on request). This has proved to be a sustainable financial model for more than 15 years, and leaves room for small investments and instrument upgrades.

    Services

    The type of service, contribution and research collaboration depend on the type of research project and the input from the research group:

    • Scientists that wish to have samples analyzed on an occasional basis, with questions that do not require extensive methods development, will usually interact directly with the technical staff of the IMSC and have their analyses done for the hourly user fee.
    • Scientists that have research questions which require extensive methods development discuss first with the head of the IMSC to define the approach and workload. Users will have to contribute financially to the methods development work at least on a partial basis and pay the regular hourly user fee for instrument usage. In the case of long-term developments (e.g. 3 months or more), which will also enhance the overall competence of the IMSC, an agreement can be made for a fixed fee according to agreed-upon criteria but will be lower than just adding the hourly user fee. It is strongly recommended that users foreseeing such a use of the IMSC discuss their project needs prior to applying for funding and include the cost in their regular project budget.
    • Scientists that have research questions, where the use of cutting-edge MS and related techniques to measure biological/clinical samples is required on a structural basis (e.g. biomarker discovery projects, systems biology projects, specific bioinformatics development and analysis) collaborate with the IMSC on a scientific level. This implies, in general, that the IMSC is already involved at the stage of the grant application and that cost for the use of instrumentation, personnel and consumables are part of the project budget. Such projects may also request dedicated instrumentation in their grants, which will be housed in the IMSC and reserved with priority for these users.
    • Scientists and commercial parties from outside the University of Groningen or the UMCG will be classified in the same way as internal academic users, but the user fees include salary cost and instrument depreciation or agreed-upon contract criteria as mentioned above.

Orbitrap Velos Pro - High resolution MS

    • Ms instrument: Orbitrap Velos Pro
    • MS Manufacturer: Thermo
    • MS Type: Linear trap-orbitrap
    • Ionization source: ESI, nanoESI
    • LC manufacturer: Thermo
    • Ms instrument: Orbitrap Velos Pro
    • MS Manufacturer: Thermo
    • MS Type: Linear trap-orbitrap
    • Ionization source: ESI, nanoESI
    • LC manufacturer: Shimadzu
    • Ms instrument: Maxis plus
    • MS Manufacturer: Bruker
    • MS Type: Quadrupole-time of flight
    • Ionization source: ESI, nanoESI
    • LC manufacturer: Thermo
    • Ms instrument: TripleTOF 6600+
    • MS Manufacturer: Sciex
    • MS Type: Quadruple-time of flight
    • Ionization source: ESI, nanoESI
    • LC manufacturer: Sciex
    • Ms instrument: Orbitrap Exploris 480
    • MS Manufacturer: Thermo
    • MS Type: Quadruple-orbitrap
    • Ionization source: ESI, nanoESI
    • LC manufacturer: Thermo
    • Ms instrument: Q Exactive Plus
    • MS Manufacturer: Thermo
    • MS Type: Quadruple-orbitrap
    • Ionization source: ESI, nanoESI
    • LC manufacturer: Thermo

Contact

Visiting address

ERIBA building, 6th floor, internal post code XB23
University of Groningen
A Deusinglaan 1
9713 AV Groningen
The Netherlands