Microscope cells

UMIC - rules and fees

Rules for using the equipment of the UMCG Microscopy and Imaging Center (UMIC) Facility
Rules for using the equipment of the UMCG Microscopy and Imaging Center (UMIC)

General rules

Always follow the instructions of our staff.

We welcome your feedback (new/improved techniques, improvements, or complaints) please contact UMIC staff at [email protected]

Acknowledge us in publications

If you present data obtained at UMIC (papers or presentations), please acknowledge the UMCG Microscopy and Imaging Center as follows:

"Part of the work has been performed at the UMCG Imaging and Microscopy Center (UMIC), which is sponsored by

if:
then:
Zeiss Supra55 ATLAS/EDX
… ZonMW grant 91111.006
Zeiss LSM7/780 confocal
… NWO 175-010-2009-023
CLEM/ Cryo-EM/SECOM
… STW Microscopy Valley 12718
Leica HPF/ FS
… NWO 91116005
TissueFAXS
… NWO 40-00506-98-9021
FAST-EM OR nanotomy data
… the Netherlands Electron Microscopy Infrastructure (NEMI; NWO 184.034.014) and EU-REACT European regional development fund as response to the COVID-19 pandemic
OMERO or LM data
… NL-BioImaging Infrastructure (NL-BI; NWO 184.036.012)
Other
… the UMCG"

 

Sent us a copy of your publication ([email protected]).

Admission and reservation

Individual training

First, register as a user and follow an individual training by UMIC staff for each instrument. The training will be provided by assigned instructors only. Training by other parties does not count!

After training UMIC staff will provide you access to the reservation system and to the facilities;

Reservations

Reservations can be made up till 2 weeks ahead (up to 3 hours slots/day)

  • Weekends, evening hours and holidays can also be reserved by experienced users
  • Microscope service/repairs always have priority
  • If reservations need to be cancelled, undo your reservation in Bookedscheduler, or ask the staff
  • If you are 15 minutes late without notification, your reservation can be cancelled

Fees

  • Users will be charged with an hourly fee No-shows will be charged the hourly fee
  • When you booking changed, please adjust your time slot in Bookedscheduler
  • It is not permitted to use any equipment without booking
  • Bills will be sent to your department on a quarterly basis

Pricelist

  tariff UMCG/RUG*
Microscope use (€/hr)
Electron Microscopy, Two-photon, Raman… 500 50
CLSM, DV, Thunder, Light sheet, LMD... 200 20
Incucyte (/plate) 10 1
Other fluorescence or Olympus BX50 100 10
Nanotomy map/sample 2000 200
ColorEM map/sample 5000 500
 
EM preparation (€/sample)
Incl. wet SEM, Embedding, (cryo)ultramicrotomy, data stoage, reagents, Except diamond knive.
Register (also wet SEM) in Logbook pc in 3215-0750
250 50
 
Spatial Omics (€/hr; 250 max/day)
Nanostring DSP, 10xGenomics Xenium 2000 50
Reagents incl. PCR machine/ run (2 slides)  3500 350
Consumables self self
 
Operator (training, experiments; €/hr) 200 70
Commercial software (Imaris) 10 5
 
*Matching included. Try to match the real costs when applying for funding of projects.

Genetically Modified Organisms (GMO) and METc

  • Indicate the use of GMOs. The number(s) of GMO permit(s) should be registered in Bookedscheduler. The rooms of the leica SP8x, CD7, LS7, zeiss780 and Stellaris are ML1. GMOs are not allowed in other areas
  • All users have to obey the regulations for ML-1 activities in their room
  • METc numbers if applicable have to be registered in Bookedscheduler

Computers and data

Users are responsible for the data they produce. Users are not allowed to install software on any of the computers on the center. The only purpose of our computers is image processing!

  • A data policy on data storage is provided at every microscope and workstation
  • Data can be stored external hard drives or USB sticks, to be provided by the users themselves
  • Data can also be stored on-line using Omero. Ask UMIC staff for access

Rules for using the incucytes

  • The Incucyte S3 may only be used by trained and registered users
  • Time-lapse intervals must be a multiple of half an hour, 30, 60, 90, 120 minutes etc
  • Booking in the UMIC-Booked Scheduler agenda is required for every plate separately
  • Plates inside an IncuCyte on a position not in Booked Scheduler can be trashedwithout notice
  • Plates inside the IncuCyte stove will be removed trashed w/o notice

Responsible use of equipment

  • Training provides you with the basic knowledge to operate a microscope.. In case of malfunctioning please contact the staff
  • Each microscope has a start-up / shut-down / stand-by instruction next to the microscope. Read this instruction and follow its steps
  • Be careful with using oil immersion and clean the spills immediately. Not all objectives can be used with oil-immersion. B A 63x lens at the LeicaSP8x and one at the Zeiss780 is a glycerol lens and should not be used with oil
  • Oil spills can be cleaned using 70% ethanol on a lenspaper. Oil is toxic. Do not! clean the lens itself with tissues, use special lenspaper for this or ask the staff

Contact

Ask us: UMIC staff
Mail us: [email protected]
Call us: +31 6 25 64 67 95