During a focus group session, eleven individuals with upper limb absence were asked about their experiences and opinions on factors influencing musculoskeletal complaints. Based on these results a preliminary overview was created with 28 factors mentioned by the participants of the focus group. With a scoping review of the literature, 39 new factors were added to the overview. This created a final overview with 67 factors divided into five main categories. The results showed that participants of the focus group predominantly mentioned psychosocial factors, such as facilities and social support from significant others and employers. The literature on the other hand, dominantly reported biomechanical factors, such as compensatory movements, posture, and increased load on the unaffected limb. Prosthesis use does not seem to have an effect on musculoskeletal complaints.