iPSC CRISPR Facility The iPSC CRISPR Facility aims to support clinical and preclinical scientists with building patient-derived iPSC models. In addition, the iPSC CRISPR Facility facilitates CRISPR genome engineering in various cell types, such as iPSCs, and also performs genome-wide CRISPR functional screens. Facility
The iPSC CRISPR Facility aims to support clinical and preclinical scientists with building patient-derived iPSC models. In addition, the iPSC CRISPR Facility facilitates CRISPR genome engineering in various cell types, such as iPSCs, and also performs genome-wide CRISPR functional screens.
This facility is an expertise centre that supports and carries out iPSC and/or CRISPR genome engineering experiments and CRISPR functional genetic screens. The facility offers, implements, and develops state-of-the-art protocols and tools that will expedite developments in regenerative medicine.

We are experts in iPSC modelling and CRISPR genome engineering. Our services include the high-throughput generation of induced pluripotent stem cells derived from patients or healthy donors. To standardize iPSC generation and increase throughput, we make use of an automated tissue culture robot. We offer researchers within the University Medical Center Groningen and the University of Groningen as well as external scientists our expertise, training, and experimental support. The facility:

  • Helps to differentiate iPSCs into various tissue types, in collaboration with other groups;
  • Introduces genetic changes using CRISPR-Cas9 technology to derive isogenic iPSC lines with targeted mutations or reporter alleles;
  • Supports genome engineering in many other cell types to generate knock-out and knock-in cell lines as well as reporter cell lines;
  • Performs genome-wide functional screens using CRISPR knock-out, inactivation, and activation libraries.
Relevance

Building improved iPSC models

The facility builds improved iPSC models and, therefore, accelerates progress in regenerative medicine. The models broaden our knowledge of the biology of iPSCs and their differentiated progeny. They also enable us to screen for modulators of their biological behaviour and to build personalized models of disease that can help physicians develop new therapeutic approaches.

Our collaborations
Together with the Netherlands Cancer Institute and Leiden University, we serve as a national infrastructure (ScreeninC) to support targeted and genome-wide CRISPR functional screens.

For more information, please visit the following websites:

Netherlands Cancer Institute; Leiden University;
national infrastructure ScreeninC.

 

Book iPSC CRISPR Facility

Please fill in the request form if you would like to make use of our facility.
Your request will be reviewed by the facility team members. You will be contacted to discuss further steps.

For the request form, click here: Request form.

Expertise and services

    • Isolation of somatic cells from patient material (blood, urine, skin);
    • Reprogramming of somatic cells (lentiviral or integration-free);
    • Characterization of iPSCs.
    • Pluripotent marker gene expression (RNA sequencing);
    • Differentiation/lineage marker gene expression (RNA sequencing);
    • Karyotyping (metaphase spreads);
    • High-resolution karyotyping (single-cell DNA sequencing).
    • Gene inactivation;
    • Gene tagging;
    • Gene alterations (such as point mutations);
    • Epigenetic modulation (CRISPRi/CRISPRa);
    • Targeted and genome-wide screens using human or mouse knock-out libraries;
    • Visualization of genomic loci;
    • Custom CRISPR applications.

Contact

European Research Institute for the Biology of Ageing

University Medical Center Groningen
Antonius Deusinglaan, 1
Building 3226
9713 AV Groningen
The Netherlands